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KMID : 0043320070300030275
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Archives of Pharmacal Research
2007 Volume.30 No. 3 p.275 ~ p.281
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Antioxidant Activity of Cleomiscosins A and C Isolated from Acer okamotoanum
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Jin WenYi
Thuong Phuong Thien
Su Nguyen Duy
Min Byung-Sun
Son Kun-Ho
Chang Hyun-Wook
Kim Hyun-Pyo
Kang Sam-Sik
Sok Dai-Eun
Bae Ki-Hwan
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Abstract
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Phytochemical investigation of Acer okamotoanum leaf and twig led to the isolation of two coumarinolignans, cleomiscosin A (1) and cleomiscosin C (2). Here, we found that 2 dose-dependently inhibits LDL oxidation mediated by either catalytic copper ions (Cu2+) or free radicals generated with the azo compound 2,2’-azobis-(2-amidinopropane)dihydro-chloride (AAPH) with IC50s of 29.5 and 11.9 킡, respectively. By electrophoretic analysis, we also observed that 2 protects apolipoprotein B-100 (apoB-100) against Cu2+-induced fragmentation (65.3% inhibition at 5 킡). Furthermore, fluorescence analyses clearly indicated that both 1 and 2 protect against the oxidative modification of apoB-100 induced by either Cu2+ or HOCl (1, IC50s of 13.4 and 8.1 킡, respectively; 2, IC50s of 23.6 and 3.9 킡, respectively). These findings suggest that 1 and 2 could be beneficial in preventing LDL oxidation in atherosclerotic lesions.
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KEYWORD
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Acer okamotoanum, Cleomiscosins A and C, Antioxidant activity, LDL Oxidation, apo-B 100 Oxidative modification
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